Journal: Materials

Article Title: Injectable Hyaluronic Acid- co -Gelatin Cryogels for Tissue-Engineering Applications

doi: 10.3390/ma11081374

Figure Lengend Snippet: Evaluation of biological properties of cryogels. Immunostaining of HAGM, MA-gelatin, HA- co -Gelatin, and MA-alginate cryogels using human CD44 His-tagged receptor and anti-gelatin antibody. CD44 binding was depicted with an Alexa Fluor 488-conjugated 6x-His Tag monoclonal antibody (green), and anti-gelatin antibody binding was detected with a FITC-conjugated anti-rabbit IgG (green) (scale bar = 100 µm).

Article Snippet: Next, cryogels were treated for 1 h at RT in the dark with Alexa Fluor 488-conjugated 6x-His Tag monoclonal antibody (Fisher Scientific) or FITC-conjugated anti-rabbit IgG (Fc-region) (Alpha Diagnostic International), respectively.

Techniques: Immunostaining, Binding Assay

Journal: Viruses

Article Title: Display of Porcine Epidemic Diarrhea Virus Spike Protein on Baculovirus to Improve Immunogenicity and Protective Efficacy

doi: 10.3390/v10070346

Figure Lengend Snippet: The organization of the expression cassette and the construction maps of the plasmids, pTriEx-S and pTriEx-S1, for generation of membrane-anchored recombinant baculovirus, S-Bac and S1-Bac. The full-length spike (S) and S1 genes are driven by the TriEx promoter, followed by the GP64 signal protein and 6xHis-tag. The pTriEx-S contains the codon optimized full-length S gene derived from the Taiwan G2b PEDV-PT strain with its original PEDV S transmembrane domain (TM-S) and the PEDV S cytoplasmic domain (CTD-S) for membrane anchoring. The pTriEx-S1 has the codon optimized S1 gene associated with GP64 transmembrane domain (TM-B) and the GP64 cytoplasmic domain (CTD-B) for membrane anchoring. Both constructs were also inserted with an mCherry fluorescent protein gene driven by the SV40-pag promoter as a reporter.

Article Snippet: The protein signals were detected by using mouse anti-6xHis-tag monoclonal antibody (1:5000 dilution, EnoGene, New York, NY, USA).

Techniques: Expressing, Membrane, Recombinant, Derivative Assay, Construct